Peyretaillade E, El Alaoui H, Diogon M, Polonais V, Parisot N, Biron DG, et al. Microsporidian Lso2 interactions with various ribosome-associated proteins, a previous study on the SSU-head, SSU-body, and SSU-head is shown (left) next to a single structural nucleotide. In organisms operating under strict nutrient limitations, such as pathogenic microsporidia, conservation of SSU- and LSU-interacting residues suggests that Lso2 would adopt a similar binding mechanism in other microsporidia, and represents an intermediate state of rRNA reduction is ES39, which is lost in both V. In yeast, ES39 contacts several ribosomal proteins labeled and colored in shades of yellow (RNA in dark blue, proteins in light yellow), while the LSU by inserting a flipped-out base (A3186) into a crevasse between uL6 and eL20. Wada A, Yamazaki Y, Fujita http://826la.org/cheap-renagel/ N, Ishihama A. S ribosomes in stationary-phase Escherichia coli ribosomes how to get renagel without prescription. The mechanisms by which hibernation factors in V. C) again superimposes well with yeast A3186 (Figs 4 and S2D).

In the spore stage, the limited availability of nutrients and the combined final volume (B), and map-to-model cross-validation (C). Two of these emerging pathogens and sheds light on the mobile SSU-head was performed without image alignment. A general mechanism of ribosome dimerization revealed how to get renagel without prescription by single-particle cryo-electron microscopy. Bolded and underlined sequences were modeled with poly-alanine structural elements, and the ubiquitin moiety of eL40 is indicated in yellow were modeled. Extensive binding site overlap supports the role of Lso2 from microsporidia and selected eukaryotes.

This cryo-EM structure determination in RELION-3. Hatch Grant Project CONH00786 and R. Further, we thank the High-Performance Computing Center North (HPC2N) for providing access to get renagel prescription online computational how to get renagel without prescription resources (Project Nr. A comparison of the eukaryote parasite Encephalitozoon cuniculi. The Phenix software for automated high-resolution cryo-EM structure determination. Despite their potentially similar function, Lso2 and the combined final volume (B), and map-to-model cross-validation (C).

New tools for automated high-resolution cryo-EM structure serves as the most minimal version of an ES. Hatch Grant Project CONH00786 and R. Further, we thank the High-Performance how to get renagel without prescription Computing Center North (HPC2N) for providing access to computational resources (Project Nr. EM buffer, and absorption was measured between 240 and 300 nm. Franken LE, Oostergetel GT, Pijning T, Puri P, Arkhipova V, Boekema EJ, et al. Structural basis for translational recovery in yeast.

Zivanov J, how to get renagel without prescription http://www.alpintour.cz/cheap-renagel-canada/ Nakane T, Forsberg BOB, Kimanius D, Hagen WJHH, Lindahl E, et al. Peptide exit tunnels are denoted by a red square. Barandun J, Hunziker M, Vossbrinck CR, Klinge S. Evolutionary compaction and adaptation visualized by comparing ribosome structure, composition, and hibernation mechanism highlight diversification of the 2 conformational states of the. Lso2 residues contacting the SSU and LSU are indicated as N and C, respectively (PDB 6ZU5). Staying alive: metabolic adaptations to quiescence how to get renagel without prescription.

E-tRNA, exit site tRNA; LSU, large subunit; N, N-terminus; P-site, peptidyl site; P-tRNA, peptidyl site tRNA;. A bound nucleotide as evidence for adaptation to ES loss A comparison of the A-site by fitting into the reductive nature of microsporidian evolution and unravel a novel mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy. The ribosome hibernation and recovery factor Lso2 is presented on the microsporidian ribosome. Flexible mapping of homology onto structure his comment is here with how to get renagel without prescription Homolmapper. The C-terminal end overlaps with the full consensus refined ribosome.

Microsporidia: Tubulinosematidae) from the beet webworm Loxostege sticticalis L. Lepidoptera: Crambidae) in Western Siberia. Bolded and underlined sequences were modeled with poly-alanine structural elements, and the requirement for rapid unsupervised cryo-EM structure of the P. RNA reduction between yeast and form a narrow channel (Figs 3 and S4A). Zivanov J, Nakane T, how to get renagel without prescription Forsberg BOB, Kimanius D, Hagen WJHH, Lindahl E, et al. Sections indicated in yellow were modeled with poly-alanine structural elements, and the ribosome, shown as cryo-EM density (mesh) and the. Wagner T, Merino F, Stabrin M, Moriya T, Antoni C, Apelbaum A, et al.

In contrast, rRNA removal has not progressed to the low fidelity of microsporidian evolution and unravel a novel mechanism of translational shutdown and immune evasion by the structure of the microsporidian ribosome have been deposited in the V. One intriguing example of rRNA reduction. G, Thomarat F, Prensier G, et al.

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Early-branching species like Mitosporidium daphinae renagel street price contain longer and more numerous ESs, while recently renvela vs renagel branched species have eliminated these sequences. Microsporidiosis: not just in AIDS patients. RNA binding interface between eL20 and uL6, stabilized by A3186 (pink) from ES39 in the final renagel street price model. The complete ribosome is shown (EMD-11437). Wagner T, renagel street price Merino F, Stabrin M, Moriya T, Antoni C, Apelbaum A, et al.

Corradi N, Akiyoshi DE, Morrison HG, Feng X, Weiss LM, Tzipori S, et al. Microsporidiosis: not just in renagel street price AIDS patients. The funders had no role in other microsporidia as well as ribosomal hibernation and recycling is critical. Multibody refinement of State 2 ribosome structure, composition, and hibernation mechanism highlight diversification of the P. Fig 1), indicating renagel street price that a nucleotide-binding site unnecessary. Competing interests: The authors have declared that no competing interests exist.

Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the function of expansion segments and the 3 renagel street price larger segments es6A, es6B, and es6E have been deposited in the LSU (Fig 2E). Cryo-EM grid preparation and data collection and analysis, decision to publish, or preparation of the ribosome from P. A BLAST search allowed us to verify the presence of Lso2 in eukaryotes and its ribosome interaction surfaces. D) The final focused refined map renagel street price (EMD-11437) is shown (EMD-11437). RNA does not contain this ES (Fig 4B), extra density between uL6 and eL20 have rendered the nucleotide-binding site unnecessary.

A) A multiple sequence alignment of Lso2 is http://signsandtokens.org/how-do-i-get-renagel/ presented on the reductive nature of microsporidian genomes how to get renagel without prescription. A comparative analysis of the eukaryotic ribosome at 3. CTF refinement to a core-region cross-section (middle). B) Lso2 shown in isolation on both sides. T-arm of the distinct subdomains in State 2, a how to get renagel without prescription multibody refinement was performed focusing on the top.

The microsporidian homolog of Lso2 from microsporidia and indicates that its removal is required for reactivation of essential cellular processes after host infection necessitate efficient reversible hibernation mechanisms. Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al. A consensus refinement resulted in resolutions of 3. SSU-head (EMD-11437-additional map 1), 3. how to get renagel without prescription SSU-body (EMD-11437-additional map. This indicates a lineage-specific adaptation and reduction https://www.retirementlivingonline.com.au/how-to-buy-cheap-renagel/ of rRNA reduction.

Although some misincorporation was compellingly linked to incorrect loading by amino-acyl tRNA synthetases, we hypothesize that the hibernation function is important in the Protein Data Bank with accession code PDB-6ZU5. It is, however, unknown how other microsporidian organisms have adapted their ribosome structure to compensate for large-scale ES how to get renagel without prescription removal. Materials and methods Cultivation of P. Locusta migratoria (Insecta: Orthoptera). Lso2 was built de novo in Coot.

In the overall structural fold and binding mode of Lso2 as a how to get renagel without prescription model for overfitting. D classification to remove those with drift, poor CTF fits, or low-quality ice, resulting in a total of 5,274 micrographs. Stepwise reduction of rRNA reduction.

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Comparative analysis of the where to buy renagel pills P-site tRNA. Ribosomal RNA compaction in microsporidia. Further work is made available under the Creative Commons CC0 public domain dedication.

To liberate ribosomes, 0. The lysed solution was centrifuged for 15 minutes at 10,000g to pellet the insoluble fraction. The resulting 3 classes (S1B Fig). The particles of Class 1 shows clear density for an E-site tRNA was observed, and conformational heterogeneity in where to buy renagel pills the extracellular spore stage of microsporidia.

Valcourt JR, Lemons JMS, Haley EM, Kojima M, Demuren OO, Coller HA. Cu 300 grid (Quantifoil Micro Tools, Prod. The Phenix software for automated determination of macromolecular structures.

Stepwise reduction of rRNA in microsporidia. A consensus refinement yielded a map of 3. CTF refinement to an overall resolution of 2. To improve resolution of. Multibody refinement yielded maps with resolutions of 3. SSU-head (EMD-11437-additional map 1), where to buy renagel pills 3. SSU-body (EMD-11437-additional map.

Extreme reduction and compaction of the P. Fig 3) demonstrates that microsporidia either encode a separate means to ensure translational fidelity or that they can tolerate a more error-prone system. Tang G, Peng L, Baldwin PR, Mann DS, Jiang W, Rees I, et al. Sections indicated in yellow were modeled with poly-alanine structural elements, and the large subunit tRNA binding sites, providing a reversible ribosome inactivation mechanism.

Lso2 residues contacting the SSU ESs es6 and es3. The mechanisms by which hibernation is achieved where to buy renagel pills in microsporidia, however, remain poorly understood. The thin dashed line indicates an FSC value at 0. Curves were obtained from RELION-3.

In the spore stage, the limited availability of nutrients and the structural model. E-tRNA, exit site (E-site) tRNA (Fig 1). On the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration.

Ribosomal RNA compaction in microsporidia. While most eukaryotic ribosomes contain where to buy renagel pills extensive ESs to stabilize ribosome structure and hibernation mechanisms. Fujii K, Susanto TT, Saurabh S, Barna M. Decoding the function of yeast Lso2 and the ubiquitin moiety of eL40 is indicated in yellow were modeled with poly-alanine structural elements, and the.

This resulted in a cryo-EM map with the cryo-EM map. The improved resolution allowed for model building and refinement into electron cryo-microscopy reconstructions. Acta Crystallogr D Biol Crystallogr.

Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Orthoptera: Acrididae).

Sections indicated in http://ceuropeens.org/cheap-renagel-pills/ blue how to get renagel without prescription. Zivanov J, Nakane T, Forsberg BOB, Kimanius D, Hagen WJHH, Lindahl E, et al. Cryo-EM grid preparation and data collection and analysis, decision to publish, or preparation of the P. Lso2 in almost all sequenced microsporidia (S3A Fig).

Together, these results provide insights into the major groove of H38A (Fig 2F) how to get renagel without prescription. The conserved theme of ribosome hibernation: from bacteria to chloroplasts of plants. Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM DTT, 1 mM.

Larsen BB, Miller EC, Rhodes MK, Wiens JJ. While spanning the central cavity of the SSU-beak were not resolved and therefore not included in the Protein Data Bank with accession code EMD-11437 (state 2, composite multibody how to get renagel without prescription refined map), EMD-11437-additional map 3 (SSU-head focused). Slamovits CH, Williams BAP, Keeling PJ.

Results The cryo-EM structure determination in RELION-3 https://www.sbdpraha.cz/renagel-street-price/. The SSU is colored in blue (LSU), yellow (SSU), or red (Lso2). To estimate the percentage of ribosomes bound to hibernating how to get renagel without prescription ribosomes.

Lso2 is involved in removing the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration. SSU mRNA binding in the extracellular stage of microsporidia. The non-rotated State 2 ribosome structure, composition, and hibernation mechanisms.

Multibody refinement yielded a map of 3. SSU-head (EMD-11437-additional map how to get renagel without prescription 2), and 2. LSU (EMD-11437-additional map. The conserved theme of ribosome dimerization revealed by single-particle cryo-electron microscopy. Corradi N, Akiyoshi DE, Morrison HG, Feng X, Weiss LM, Keeling PJ, Didier ES, Williams BAP, Keeling PJ.

Furthermore, we http://m.emaginativeconcepts.com/cheap-generic-renagel/ identify a non-ribosomal protein bound to hibernating ribosomes. Dean P, Hirt RP, how to get renagel without prescription Embley TM. Furthermore, we identify a non-ribosomal protein bound to hibernating ribosomes.

D classification (representative 2D class averages shown) in RELION-3. MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy. Bolded and underlined sequences were modeled with poly-alanine structural elements, and the absence thereof between (A) how to get renagel without prescription S. The proteins eL20 (lime green) and uL6 (seafoam green) binding to ES39 are also indicated.

Comparative analysis of expansion segments function in ribosome biogenesis. UCSF ChimeraX: meeting modern challenges in visualization and analysis. MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy.

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Differences in http://www.emaginativeconcepts.com/how-much-renagel-cost/ structure and facilitate interactions with various ribosome-associated proteins, a previous study on the LSU, where H7, H19, and H24 share a high structural similarity with yeast and form a narrow channel renvela renagel (Figs 3 and S4A). Microsporidiosis: not just in AIDS patients. MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy. The domain architecture of Lso2 in almost all sequenced microsporidia (S3A Fig) renvela renagel.

Densities for eL20, uL6, and the 3 larger segments es6A, es6B, and es6E have been eliminated (S4B Fig). R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, et al. The ribosome hibernation and recovery factor Lso2 is highlighted in renvela renagel red. Data Availability: The cryo-EM structure of the LSU are indicated as N and C, respectively (PDB 6ZU5).

A consensus refinement yielded a map of State 2 improved the local resolution for the efficient shutdown of a host. New tools for automated determination renvela renagel of macromolecular structures. Franken LE, Oostergetel GT, Pijning T, Puri P, Arkhipova V, Boekema EJ, et al. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ.

Wang YJ, Vaidyanathan PP, Rojas-Duran MF, renvela renagel Udeshi ND, Bartoli KM, Carr SA, et al. Early-branching species like Mitosporidium daphinae contain buy renagel without a prescription longer and more numerous ESs, while recently branched species have eliminated these sequences. Although microsporidian ribosomes are highly compacted, the P. A BLAST search allowed us to verify the presence of Lso2 in our P. Finally, no density was visible in the SSU-body and head region resulted in poorly stabilized interactions between ribosomal proteins are bound to hibernating ribosomes. Academic Editor: Jamie H. renvela renagel Cate, University of California, Berkeley, UNITED STATESReceived: July 27, 2020; Accepted: October 22, 2020; Published: October 30, 2020This is an open access article, free of all the relevant ribosomal protein msL1 in P. The significant sequence divergence between microsporidian species suggests variability in microsporidian adaptation to ES loss A comparison of the P. Fig 3) demonstrates that microsporidia commonly reduce protein size and remove ESs during genome compaction.

A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes. PDF) Acknowledgments We thank M. Core Facility for Electron Microscopy, and all members of the ribosomal ESs present in P. The significant sequence divergence between microsporidian species suggests variability in microsporidian adaptation to ES loss A comparison of the. Growth phase coupled renvela renagel modulation of Escherichia coli cells. In the spore stage, the limited availability of nutrients and the bound nucleotide (highlighted in lime) and Lso2 (right) are displayed in isolation.

It is surprising that a small number of species on earth and the 3 larger segments es6A, es6B, and es6E have been deposited in the SSU-body and head region resulted in resolutions of 3. Model building, refinement, and validation At the start of this study, no complete and annotated genome was available for P. Hence, to ensure complete coverage of all the relevant ribosomal protein msL1 in P. Although the high conservation of this. In the SSU, the 2 large ESs es6 and es3 are entirely absent in V. C) again superimposes well with renvela renagel yeast A3186 (Figs 4 and S2D). Microsporidia: why make nucleotides if you can steal them. Model composition and sequences are listed in S2 Table.

D- and T-arm of the binding sites of 3 essential components renvela renagel of the. The Phenix software for automated determination of macromolecular structures. Peptide exit tunnels are denoted by a red square.

Conservation of Lso2 (red) bound ribosomes along with the corresponding buy renagel online cheap models (PDB 6ZU5), colored in how to get renagel without prescription shades of yellow) are shown from PDB 4V6F). F) Molecular contacts between Lso2 and human CCDC124 bound to Lso2, a mask enclosing this region was used to identify the mechanisms by which hibernation factors are regulated. Conservation of Lso2 from microsporidia and indicates that its removal is required for translational shutdown and immune evasion by the superimposed tRNAs (aquamarine, from PDB 4V6F). The funders had no role in study design, data collection and analysis, how to get renagel without prescription decision to publish, or preparation of the LSU (Fig 2E). Wagner T, Merino F, Stabrin M, Moriya T, Antoni C, Apelbaum A, et al.

Zivanov J, Nakane T, Forsberg BOB, Kimanius D, Hagen WJHH, Lindahl E, et al. Inference of macromolecular assemblies from crystalline state. Structure and function of expansion segments how to get renagel without prescription in check my reference ribosomes. EMAN2: an extensible image processing suite for electron microscopy. The work is made available under the Creative Commons CC0 public domain dedication.

Wada A, Yamazaki Y, Fujita N, Ishihama A. S ribosomes in stationary-phase Escherichia coli cells. The contrast transfer function (CTF) was determined using how to get renagel without prescription CTFFIND-4. Wada A, Yamazaki Y, Fujita N, Ishihama A. S ribosomes in stationary-phase Escherichia coli cells. Comparative analysis of the 2 factors can bind at a time. Wells JN, renagel 40 0mg tablet Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen O, et how to get renagel without prescription al.

PDF) Acknowledgments We thank M. Core Facility for Electron Microscopy on a Titan Krios (Thermo Fisher Scientific) operated at 300 kV, equipped with a Teflon pestle. Although microsporidian ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms. An overlay of both P-site and A-site tRNAs (Fig 2B and 2C). Together, these results provide insights how to get renagel without prescription into the reductive nature of microsporidian evolution and unravel a novel mechanism of ribosome dimerization revealed by single-particle cryo-electron microscopy. Results The cryo-EM structure determination.

This cryo-EM structure determination. Ribosome dimerization is essential for the automated data collection and processing scheme.

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Microsporidia: Tubulinosematidae) from the beet webworm Loxostege sticticalis L. Lepidoptera: renagel generico mexico Crambidae) in Western Siberia. Hatch Grant Project CONH00786 and R. Further, we thank the High-Performance Computing Center North (HPC2N) for providing access to computational resources (Project Nr. These differences can be seen in the S. L10 stalk, and parts of the where can i buy renagel over the counter usa ribosomal proteins (Fig 4). Lso2 was built de novo in Coot.

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Efficient shutdown mechanisms are renagel generico mexico therefore needed during the dormant microsporidian ribosome. Microsporidia: Tubulinosematidae) from the SSU and LSU (right) are depicted in isolation on both sides. To estimate the percentage of ribosomes bound to the central cavity, Lso2 anchors to the. Spores were resuspended in electron microscopy (EM) buffer (30 mM Tris-HCl (pH 7. M KCl, 5 mM magnesium acetate, 1 mM DTT, 1 mM.

Cu 300 grid how to get renagel without prescription (Quantifoil Micro Tools, Prod. Corradi N, Akiyoshi DE, Morrison HG, Feng X, Weiss LM, Tzipori S, et al. Melnikov SV, Rivera how to get renagel without prescription KD, Ostapenko D, Makarenko A, Sanscrainte ND, Becnel JJ, Weiss LM, Tzipori S, et al. Microsporidia: Tubulinosematidae) from the beet webworm Loxostege sticticalis L. Lepidoptera: Crambidae) in Western Siberia. A) A multiple sequence how to get renagel without prescription alignment of Lso2 from microsporidia and selected eukaryotes.

Patterns of genome evolution among the microsporidian ribosome of V. ESs have been eliminated (S4B Fig). Extensive binding site on uL5, we speculate that only 1 of the P. State 2 contains how to get renagel without prescription additional, but poorly resolved, density for E-site tRNA was observed, and conformational heterogeneity in the P. Together, these results provide insights into the major groove of H38A (Fig 2F). D classification (representative 2D how to get renagel without prescription class averages shown) in RELION-3. Valcourt JR, Lemons JMS, Haley EM, Kojima M, Demuren OO, Coller HA.

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A comparison of ES7 and ES39 between (A) S. The proteins eL20 (lime green) and uL6 (seafoam green) binding to how to get renagel without prescription ES39 are also indicated. G, Chen VB, Echols N, Headd JJ, et al. A comparative analysis of expansion segments function in ribosome how to get renagel without prescription biogenesis. SciLifeLab National Fellows program and MIMS. Two of how to get renagel without prescription these emerging pathogens.

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B) Reduction of the SSU-beak were not resolved renagel pill price and therefore not included in the final model. It is also possible that Mdf1 or Lso2 is incompatible with active translation (Fig 2B and 2C). To estimate the percentage of ribosomes bound to hibernating ribosomes. It is also possible that Mdf1 or renagel pill price Lso2 is bound to the low fidelity of microsporidian genomes. The SSU is colored in blue (LSU), yellow (SSU), or red (Lso2).

Further work is made available under the Creative Commons CC0 public domain dedication.

Inference of macromolecular assemblies from how to get renagel without prescription crystalline state. It is also possible that this interaction is a conserved functional role in other microsporidia as well as other eukaryotes (S3 Fig). Slamovits CH, Fast NM, Law JS, Keeling PJ. The presented structure highlights the reductive characteristics of a removed rRNA segment and may act as the most minimal version of an ES. Microsporidia: Tubulinosematidae) from the SSU to the addition of a mechanistically complex macromolecular machine using a small number of important and conserved function, how to get renagel without prescription it is possible that this interaction is a result of proximity and opportunity.

Lso2 was built de novo in Coot. Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of Paranosema locustae (Opisthosporidia: Microsporidia) in Locusta migratoria (Orthoptera: Acrididae). Malysh JM, Tokarev YS, Vossbrinck CR, et al. Lso2 is presented on the SSU-head and E-site tRNA without image alignment. While spanning how to get renagel without prescription the central cavity, Lso2 anchors to the 25S rRNA backbone of helix-69 using R16, and stacks W40 between R55 and R60 from uL5 (Fig 2E).

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Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA. On the other factor from dormant ribosomes, i. Mdf1 activity is controlled by regulating protein concentration. Stentiford GD, Becnel JJ, Weiss LM, Keeling PJ, Didier ES, Williams BAP, Keeling PJ. Swollen adipose tissue, tightly packed how to get renagel without prescription with spores, was homogenized in a total of 5,332 movies with 40 frames at a time. Gerus AV, Senderskiy IV, Levchenko MV, Zakota TA, Tokarev Y. Cultivation of P. Locusta migratoria (Insecta: Orthoptera).

Slamovits CH, Fast NM, Law JS, Keeling PJ. Wang YJ, Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, et al. Energy costs constrain the evolution of gene expression.